SGsystem’s SG-PCR Premix-EX is an optimized mixture composed of enzyme, reaction buffer and dNTP mixture at a 2-fold concentration. SG-PCR Premix-EX is designed to allow the user quick and easy reaction assembly of the reaction. Users should adjust a final reaction to 1x (ex. For 20ul PCR reaction, dispense by 10ul each tube). This enzyme performance is confirmed by successful amplification of a 10 kb lambda DNA template. PCR products amplified with SG-PCR DNA Polymerase have 3’-dA overhangs and blunt ends.
● High yield or high sensitivity PCR
● PCR products generated with SG-PCR DNA Polymerase contain a mixture of 3’-dA overhangs and blunt ends.
● Max size : 10 kbp
● Ready-to-use formula
● Store at -20 ℃
● Repeated freeze-thaw cycles may decrease the enzyme activity.
● SG-PCR Premix-EX is stable for at least 2 years at recommended storage condition
General reaction mixture for PCR (total 20 ul)
SG-PCR Premix-EX : 10 ul
Template : 0.1-10 ng
Primer 1(10 pmol/ul) : 0.5 ul
Primer 2(10 pmol/ul) : 0.5 ul
Sterilized distilled water : up to 20 ul
Initial denature at 95 ℃ : 3 min
95 ℃ 30 sec
Tm-4℃ 30 sec
72℃ 30-60 sec/kbp
* 25~45 PCR cycles
1 ㎖ (100rxn) 5EA
10ul/ 20ul reaction
2x Ready-to-use mix